recombinant human notch1 protein Search Results


notch 1  (R&D Systems)
94
R&D Systems notch 1
Notch 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/notch 1/product/R&D Systems
Average 94 stars, based on 1 article reviews
notch 1 - by Bioz Stars, 2026-03
94/100 stars
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recombinant notch1 extracellular domain  (R&D Systems)
93
R&D Systems recombinant notch1 extracellular domain
Recombinant Notch1 Extracellular Domain, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant notch1 extracellular domain/product/R&D Systems
Average 93 stars, based on 1 article reviews
recombinant notch1 extracellular domain - by Bioz Stars, 2026-03
93/100 stars
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notch1  (OriGene)
92
OriGene notch1
A) Western blot of in vitro cleavage of recombinant cadherin with purified 3CLPro. 1uM of purified 3CLPro was incubated with 2ug of recombinant CDH6 or CDH20 (C-terminal His-Tag) in a 50uL reaction for 1hr. Shown are cleavage sites within the recombinant fragment, with amino acid positions displayed for the full length proteins. Western blots showing staining against the C-terminus (His-Tag) of each protein and 3CLPro. Recombinant proteins are a mixture of glycosylated (∼90kDa) and unglycosylated (∼65kda), corresponding to cleavage fragments of ∼62kDa and 40kDa (respectively). B) Western blot of in vitro cleavage of purified human alpha thrombin (IIa). Diagram shows amino acid position of unprocessed prothrombin. Position of cleavage site shown with respect to epitope of antibody used for western blot. 1uM of purified 3CLpro was incubated with 2ug alpha thrombin overnight under reducing conditions, with or without the 3CLPro inhibitor GC376 (1uM). C) In vitro cleavage of purified recombinant <t>NOTCH1</t> fragment (aa2280-2550) with a N-terminal His-Tag. Reactions were done with 1uM of purified 3CLPro for 1hr. Diagram shows position of cleavage within the NOTCH1 fragment, with amino acid positions corresponding to the full length protein. Epitope regions showed for antibody with epitope C-terminal to the cleavage site. Full length size is ∼29kDa, with N and C-terminal fragments of 4kDa and 25kDa (respectively). D) Cellular cleavage of NOTCH1. Western blots show lysates of hIPSC cardiomyocytes expressing 3CLPro or catalytically inactive C145A variant for 48h. Cleavage site position within the intracellular fragment of NOTCH1 shown, as well as epitope for antibody used in western blot. For all statistics shown, * = p ≤ 0.05 , ** p ≤ 0.01, ***p ≤ 0.001, **** = p ≤ 0.00001
Notch1, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/notch1/product/OriGene
Average 92 stars, based on 1 article reviews
notch1 - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier

Image Search Results


A) Western blot of in vitro cleavage of recombinant cadherin with purified 3CLPro. 1uM of purified 3CLPro was incubated with 2ug of recombinant CDH6 or CDH20 (C-terminal His-Tag) in a 50uL reaction for 1hr. Shown are cleavage sites within the recombinant fragment, with amino acid positions displayed for the full length proteins. Western blots showing staining against the C-terminus (His-Tag) of each protein and 3CLPro. Recombinant proteins are a mixture of glycosylated (∼90kDa) and unglycosylated (∼65kda), corresponding to cleavage fragments of ∼62kDa and 40kDa (respectively). B) Western blot of in vitro cleavage of purified human alpha thrombin (IIa). Diagram shows amino acid position of unprocessed prothrombin. Position of cleavage site shown with respect to epitope of antibody used for western blot. 1uM of purified 3CLpro was incubated with 2ug alpha thrombin overnight under reducing conditions, with or without the 3CLPro inhibitor GC376 (1uM). C) In vitro cleavage of purified recombinant NOTCH1 fragment (aa2280-2550) with a N-terminal His-Tag. Reactions were done with 1uM of purified 3CLPro for 1hr. Diagram shows position of cleavage within the NOTCH1 fragment, with amino acid positions corresponding to the full length protein. Epitope regions showed for antibody with epitope C-terminal to the cleavage site. Full length size is ∼29kDa, with N and C-terminal fragments of 4kDa and 25kDa (respectively). D) Cellular cleavage of NOTCH1. Western blots show lysates of hIPSC cardiomyocytes expressing 3CLPro or catalytically inactive C145A variant for 48h. Cleavage site position within the intracellular fragment of NOTCH1 shown, as well as epitope for antibody used in western blot. For all statistics shown, * = p ≤ 0.05 , ** p ≤ 0.01, ***p ≤ 0.001, **** = p ≤ 0.00001

Journal: bioRxiv

Article Title: Prediction and validation of host cleavage targets of SARS-CoV-2 3C-like protease

doi: 10.1101/2022.01.17.476677

Figure Lengend Snippet: A) Western blot of in vitro cleavage of recombinant cadherin with purified 3CLPro. 1uM of purified 3CLPro was incubated with 2ug of recombinant CDH6 or CDH20 (C-terminal His-Tag) in a 50uL reaction for 1hr. Shown are cleavage sites within the recombinant fragment, with amino acid positions displayed for the full length proteins. Western blots showing staining against the C-terminus (His-Tag) of each protein and 3CLPro. Recombinant proteins are a mixture of glycosylated (∼90kDa) and unglycosylated (∼65kda), corresponding to cleavage fragments of ∼62kDa and 40kDa (respectively). B) Western blot of in vitro cleavage of purified human alpha thrombin (IIa). Diagram shows amino acid position of unprocessed prothrombin. Position of cleavage site shown with respect to epitope of antibody used for western blot. 1uM of purified 3CLpro was incubated with 2ug alpha thrombin overnight under reducing conditions, with or without the 3CLPro inhibitor GC376 (1uM). C) In vitro cleavage of purified recombinant NOTCH1 fragment (aa2280-2550) with a N-terminal His-Tag. Reactions were done with 1uM of purified 3CLPro for 1hr. Diagram shows position of cleavage within the NOTCH1 fragment, with amino acid positions corresponding to the full length protein. Epitope regions showed for antibody with epitope C-terminal to the cleavage site. Full length size is ∼29kDa, with N and C-terminal fragments of 4kDa and 25kDa (respectively). D) Cellular cleavage of NOTCH1. Western blots show lysates of hIPSC cardiomyocytes expressing 3CLPro or catalytically inactive C145A variant for 48h. Cleavage site position within the intracellular fragment of NOTCH1 shown, as well as epitope for antibody used in western blot. For all statistics shown, * = p ≤ 0.05 , ** p ≤ 0.01, ***p ≤ 0.001, **** = p ≤ 0.00001

Article Snippet: Recombinant proteins were purchased commercially: NOTCH1 (Origene, Cat# TP762041), CDH6 (ACROBiosystem, Cat# CA6-H5229), CDH20 (R&D, Cat# 5604-CA-050).

Techniques: Western Blot, In Vitro, Recombinant, Purification, Incubation, Staining, Expressing, Variant Assay

A) Western blots of candidate cleavage targets. 48h overexpression of 3CLPro or C145A in 293T cells. B) Western blot for the C-terminal cleavage fragment of NOTCH1 following 48h overexpression in 293T cells.

Journal: bioRxiv

Article Title: Prediction and validation of host cleavage targets of SARS-CoV-2 3C-like protease

doi: 10.1101/2022.01.17.476677

Figure Lengend Snippet: A) Western blots of candidate cleavage targets. 48h overexpression of 3CLPro or C145A in 293T cells. B) Western blot for the C-terminal cleavage fragment of NOTCH1 following 48h overexpression in 293T cells.

Article Snippet: Recombinant proteins were purchased commercially: NOTCH1 (Origene, Cat# TP762041), CDH6 (ACROBiosystem, Cat# CA6-H5229), CDH20 (R&D, Cat# 5604-CA-050).

Techniques: Western Blot, Over Expression